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Year 2005, Month 5 (Section Full Articles, Page 1298)

Reversion of the multiple-drug resistance phenotype mediated by short interfering RNAs

E. B. Logashenko, A. V. Vladimirova, A. N. Zenkov, M. N. Repkova, A. G. Ven´yaminova, E. L. Chernolovskaya,* and V. V. Vlassov

Repression of MDR1 gene expression and restoration of cancer cell sensitivity to cytostatic agents were studied with the use of synthetic siRNAs directed to different MDR1 mRNA regions. Short interfering RNAs that enhance sensitivity of drug-resistant cells to vinblastine were revealed. The ability of various siRNAs to repress gene expression does not correlate with the efficiency of antisense oligonucleotides complementary to the same mRNA regions. An siRNA sample remained intact in a medium during a period of time sufficient to exhibit biological activity. The results of our study suggest that siRNAs can be considered as a basis for therapeutic drugs for enhancement of the efficiency of antitumor chemotherapy.

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