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ISSUE 5

Year 2005, Month 5 (Section Full Articles, Page 1306)

Effect of DNA and bivalent metal ions on the interaction of thermostable DNA polymerase Tte with dNTPs

E. A. Belousova,(a) V. V. Koval,(a) N. I. Rechkunova,(a) S. Kh. Degtyarev,(b) O. S. Fedorova,(a) and O. I. Lavrik(a)*

The interaction of DNA polymerase Tte from Thermus thermophilus B35 with dUTP analog containing a fluorescein residue bound to C(5) of the base (Flu-dUTP) was studied by fluores- cence titration. The dissociation constants of the enzyme—substrate complexes in the absence and in the presence of a DNA duplex containing an extended template and bivalent metal ions and the kinetic parameters of polymerization by DNA polymerase Tte in the presence of Flu-dUTP were determined

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